TAP1 Monoklonaler Antikörper

TAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B8A7

Kat-Nr. : 68412-1-PBS

Synonyme

ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3

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  WB analysis using 68412-1-Ig (same clone as 68412-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68412-1-Ig (TAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68412-1-Ig (same clone as 68412-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TAP1 antibody (68412-1-Ig, Clone: 1B8A7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68412-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TAP1 fusion protein Ag21303
• Vollständiger Name: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC014081
• Gene symbol: TAP1
• Gene ID (NCBI): 6890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70 kDa.