TAP1 Rekombinanter Antikörper

TAP1 Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

250037E7

Kat-Nr. : 85766-4-RR

Synonyme

ABCB2, ATP-binding cassette sub-family B member 2, EC:7.4.2.14, PSF1, PSF-1

---

• 

  WB analysis using 85766-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 85766-4-RR

  THP-1 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 85766-4-RR

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 85766-4-RR (TAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 85766-4-RR

  IP result of anti-TAP1 (IP:85766-4-RR, 4ug; Detection:85766-4-RR 1:1000) with HepG2 cells lysate 1800 ug.

• 

  IHC staining of human pancreas cancer using 85766-4-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 85766-4-RR (TAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85766-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85766-4-RR against Human TAP1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HCT 116-Zellen, HepG2-Zellen, Mausmilzgewebe, THP-1-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85766-4-RR bindet in WB, IHC, IP, ELISA TAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TAP1 fusion protein Ag1619
• Vollständiger Name: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC014081
• Gene symbol: TAP1
• Gene ID (NCBI): 6890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70-80 kDa in HeLa cells.