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TAPBP Polyklonaler Antikörper

TAPBP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30500-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using TAPBP Polyclonal antibody (30500-1-AP)

    WB analysis using 30500-1-AP (same clone as 30500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30500-1-AP (TAPBP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TAPBP Polyclonal antibody (30500-1-AP)

    IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using TAPBP Polyclonal antibody (30500-1-AP)

    IHC staining of mouse colon using 30500-1-AP (same clone as 30500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30500-1-AP (TAPBP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TAPBP Polyclonal antibody (30500-1-AP)

    IF Staining of HeLa using 30500-1-AP (same clone as 30500-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TAPBP antibody (30500-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30500-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30500-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TAPBP und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TAPBP fusion protein Ag30885
Vollständiger Name TAP binding protein (tapasin)
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht48 kDa
GenBank-ZugangsnummerNM_003190
Gene symbol TAPBP
Gene ID (NCBI) 6892
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAPBP, also known as tapasin, is a V-C1 (variable-constant) immunoglobulin superfamily (IgSF) molecule. It is involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (PMID: 11920573).