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TDP-43 (C-terminal) Polyklonaler Antikörper
TDP-43 (C-terminal) Polyklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (4)
Anwendung
WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, chIP, RIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 12892-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A549-Zellen, C6-Zellen, HeLa-Zellen, K-562-Zellen, Maushirngewebe |
Erfolgreiche IP | Maushirngewebe |
Erfolgreiche Detektion in IHC | Rattenhirngewebe, humanes Gliomgewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-Fro | Maushirngewebe |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, Neuro-2a-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF)-FRO | IF-FRO : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:2000-1:8000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
Produktinformation
12892-1-AP bindet in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, chIP, RIP, ELISA TDP-43 (C-terminal) und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, drosophila, Huhn, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Fusionsprotein |
Vollständiger Name | TAR DNA binding protein |
Berechnetes Molekulargewicht | 43 kDa |
Beobachtetes Molekulargewicht | 43-45 kDa, 35 kDa |
GenBank-Zugangsnummer | BC001487 |
Gene symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Protokoll herunterladen |
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Protokoll herunterladenl |
IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Protokoll herunterladen |
IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Science HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.
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Nature Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. | ||
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emilie (Verified Customer) (09-24-2025) | I used it both in WB (1:1000) and IF (1:200), and it performed as expected.
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FH Manon (Verified Customer) (09-23-2025) | The antibody produced clear, specific staining by immunofluorescence and also detected the protein at the expected size by western blot.
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FH Xiaochen (Verified Customer) (11-11-2024) | sensitive and specific
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FH Scott (Verified Customer) (10-22-2024) | 10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size, blue bar, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.
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FH Parijat (Verified Customer) (09-09-2023) | Good antibody
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FH Xin (Verified Customer) (01-23-2022) | Good performance in WB (around 45 kD)
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FH Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by TDP-43 (C-terminal) Polyclonal antibody at dilution of 1:500 showed strong labelling.
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FH Jacob (Verified Customer) (03-25-2021) | Good for IF staining. It worked for cells fixation with 4% PFA.
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FH David (Verified Customer) (01-13-2020) | Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.
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FH Alinda (Verified Customer) (02-28-2019) | Good antibody
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FH Noemi (Verified Customer) (01-24-2019) | really good antibody, it detects also the cleavage product of TDP43 in WB.
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