TDP-43 (full length) Polyklonaler Antikörper
TDP-43 (full length) Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18280-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | K-562-Zellen |
Erfolgreiche IP | K-562-Zellen |
Erfolgreiche Detektion in IHC | Maushirngewebe, humanes Gliomgewebe, Rattenhirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | SH-SY5Y-Zellen |
Erfolgreiche Detektion in FC (Intra) | SH-SY5Y-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:200-1:2000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:1500-1:6000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 5 publications below |
IHC | See 6 publications below |
IF | See 5 publications below |
IP | See 2 publications below |
Produktinformation
18280-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA TDP-43 (full length) und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Fusionsprotein |
Vollständiger Name | TAR DNA binding protein |
Berechnetes Molekulargewicht | 43 kDa |
Beobachtetes Molekulargewicht | 43 kDa |
GenBank-Zugangsnummer | BC001487 |
Gene symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for TDP-43 (full length) antibody 18280-1-AP | Protokoll herunterladen |
IHC protocol for TDP-43 (full length) antibody 18280-1-AP | Protokoll herunterladenl |
IF protocol for TDP-43 (full length) antibody 18280-1-AP | Protokoll herunterladen |
IP protocol for TDP-43 (full length) antibody 18280-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Neurosci TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates. | ||
Acta Neuropathol Commun Anatomic survey of seeding in Alzheimer's disease brains reveals unexpected patterns. | ||
Neurobiol Dis Tau-tubulin kinase 1 phosphorylates TDP-43 at disease-relevant sites and exacerbates TDP-43 pathology | ||
Dis Model Mech Viral delivery of C9ORF72 hexanucleotide repeat expansions in mice lead to repeat length dependent neuropathology and behavioral deficits. | ||
J Neurotrauma Multimodal Biomarkers of Repetitive Head Impacts and Traumatic Encephalopathy Syndrome: A Clinico-Pathological Case Series. | ||
J Alzheimers Dis Pathological Correlates of Cognitive Impairment in The University of Manchester Longitudinal Study of Cognition in Normal Healthy Old Age. |