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TARS Monoklonaler Antikörper

TARS Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1A11F10

Kat-Nr. : CL594-67828

Synonyme

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated TARS Monoclonal antibody (CL594-67828)

    IF Staining of HeLa using CL594-67828

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 TARS antibody (CL594-67828, Clone: 1A11F10 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67828 bindet in IF/ICC TARS und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen TARS fusion protein Ag6614
Vollständiger Name threonyl-tRNA synthetase
Berechnetes Molekulargewicht 83 kDa
Beobachtetes Molekulargewicht 83-87 kDa
GenBank-ZugangsnummerBC000517
Gene symbol TARS
Gene ID (NCBI) 6897
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 TARS antibody CL594-67828Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen