TASOR Polyklonaler Antikörper

TASOR Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (3)

Kat-Nr. : 30188-1-AP

Synonyme

C3orf63, FAM208A, se89 1, RAP140, KIAA1105

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  WB analysis using 30188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30188-1-AP (TASOR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30188-1-AP (TASOR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 30188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 30188-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30188-1-AP (TASOR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30188-1-AP bindet in WB, IHC, ELISA TASOR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TASOR fusion protein Ag32757
• Vollständiger Name: chromosome 3 open reading frame 63
• Berechnetes Molekulargewicht: 189 kDa
• Beobachtetes Molekulargewicht: 189 kDa
• GenBank-Zugangsnummer: NM_001112736.1
• Gene symbol: TASOR
• Gene ID (NCBI): 23272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TASOR, also known as FAM208A or C3orf63, is the central subunit of the human silencing hub (HUSH) complex. HUSH regulates deposition of the epigenetic mark H3K9me3. TASOR has a catalytically-inactive PARP domain which is necessary for targeted H3K9me3 deposition (PMID: 33009411). TASOR and the RNA deadenylase CCR4-NOT complex scaffold CNOT1 synergistically repress HIV proviral expression (PMID: 35013187). TASOR affects the genome stability during the cellular division and also interacts with methylation complex in adult tissues (PMID: 34272044).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (09-22-2025)

Antibody recognises the band of the expected size as well as several non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: U2OS

TASOR Antibody Western Blot validation (1:1500 dilution) in U2OS (Cat no:30188-1-AP)

FH

Julia (Verified Customer) (07-02-2025)

Works well! Validated with KO.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESC

TASOR Antibody Western Blot validation (1:1000 dilution) in mESC (Cat no:30188-1-AP)

FH

Roy (Verified Customer) (06-12-2024)

Could detect TASOR in HeLa cells and Jurkat cells (1/1000 dilution - Overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa, Jurkat