TAU Monoklonaler Antikörper

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• KD/KO Validated

TAU Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E9A8

Kat-Nr. : 66499-1-PBS

Synonyme

MAPT, 1E9A8, AT180, DDPAC, MAPTL

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  WB analysis of HeLa using 66499-1-Ig (same clone as 66499-1-PBS)

  WB result of TAU antibody (66499-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAU transfected HeLa cells. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  WB analysis using 66499-1-Ig (same clone as 66499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66499-1-Ig (TAU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66499-1-Ig (same clone as 66499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66499-1-Ig (TAU antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66499-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66499-1-PBS bindet in WB, IHC, Indirect ELISA TAU und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2c
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TAU fusion protein Ag21926
• Vollständiger Name: microtubule-associated protein tau
• Berechnetes Molekulargewicht: 37-46, 79-81 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC000558
• Gene symbol: TAU
• Gene ID (NCBI): 4137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (PMID:8752131,15965697, 12485403).Present monoclonal anti-Tau antibody can detect approx 100-kDa bands in brain tissues.