TAX1BP1 Polyklonaler Antikörper

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• KD/KO Validated

TAX1BP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Kat-Nr. : 14424-1-AP

Synonyme

TRAF6-binding protein, Tax1-binding protein 1, T6BP, PRO0105

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  WB analysis using 14424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14424-1-AP (TAX1BP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 14424-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 14424-1-AP (TAX1BP1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14424-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TAX1BP1 antibody (14424-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IF: See 6 publications below
• IP: See 2 publications below

Produktinformation

14424-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TAX1BP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAX1BP1 fusion protein Ag5789
• Vollständiger Name: Tax1 (human T-cell leukemia virus type I) binding protein 1
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC050358
• Gene symbol: TAX1BP1
• Gene ID (NCBI): 8887
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAX1BP1 (also named as T6BP and TXBP151) inhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. It is degraded by caspase-3-like family proteins upon TNF-induced apoptosis. TAX1BP1 may also play a role in the pro-inflammatory cytokine IL-1 signaling cascade. TAX1BP1 binds TRAF6 but not TRAFs 1-5.

Publikationen

Species	Application	Title
mouse	WB	Cell; NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.; Authors - Zhenyu Zhong
human	WB	Nat Commun; Activation of lysophagy by a TBK1-SCFFBXO3-TMEM192-TAX1BP1 axis in response to lysosomal damage; Authors - Na Yeon Park
	WB	Mol Cell; PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis; Authors - Shouqiao Hou
mouse	WB	Redox Biol; A detrimental role of NLRP6 in host iron metabolism during Salmonella infection.; Authors - Qifeng Deng
mouse	WB	Autophagy; TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy.; Authors - Wenxian Wu
human	IF	Autophagy; Inhibition of the SEC61 translocon by mycolactone induces a protective autophagic response controlled by EIF2S1-dependent translation that does not require ULK1 activity.; Authors - Belinda S Hall