TBC1D1 Polyklonaler Antikörper

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• KD/KO Validated

TBC1D1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Rezensionen (1)

Kat-Nr. : 22124-1-AP

Synonyme

KIAA1108, TBC, TBC1, TBC1 domain family member 1, TBC1D1

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  WB analysis of LNCaP using 22124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 22124-1-AP

  WB result of TBC1D1 antibody (22124-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D1 transfected HEK-293T cells.

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  WB analysis using 22124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22124-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22124-1-AP (TBC1D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 22124-1-AP

  IP result of anti-TBC1D1 (IP:22124-1-AP, 4ug; Detection:22124-1-AP 1:1000) with Jurkat cells lysate 2400ug.

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  IHC staining of human testis using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22124-1-AP (TBC1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 22124-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22124-1-AP (TBC1D1 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

22124-1-AP bindet in WB, IHC, IP, ELISA TBC1D1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBC1D1 fusion protein Ag5406
• Vollständiger Name: TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
• Berechnetes Molekulargewicht: 133 kDa
• Beobachtetes Molekulargewicht: 150-165 kDa
• GenBank-Zugangsnummer: BC050321
• Gene symbol: TBC1D1
• Gene ID (NCBI): 23216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Metab; Skeletal muscle from TBC1D4 p.Arg684Ter variant carriers is severely insulin resistant but exhibits normal metabolic responses during exercise; Authors - Jonas M Kristensen
human	WB,IP	Diabetes; Illumination of the Endogenous Insulin-Regulated TBC1D4 Interactome in Human Skeletal Muscle.; Authors - Jeppe K Larsen
mouse	WB	Mol Nutr Food Res; Quercetin oppositely regulates insulin-mediated glucose disposal in skeletal muscle under normal and inflammatory conditions: The dual roles of AMPK activation.; Authors - Kang Liu
human	WB	J Physiol; The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres.; Authors - Magnus R Larsen
		J Biol Chem; Cooperative actions of Tbc1d1 and AS160/Tbc1d4 in GLUT4 trafficking activities.; Authors - Hiroyasu Hatakeyama
mouse	WB	J Pharmacol Sci; PMID: 30733181; Authors - Zejun Mo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Q (Verified Customer) (06-01-2021)

Excellent to detect TBC1D1 in postmortem human samples with WB with the right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: postmortem human brain lysate

TBC1D1 Antibody Western Blot validation (1:2000 dilution) in postmortem human brain lysate (Cat no:22124-1-AP)