TBC1D17 Polyklonaler Antikörper

TBC1D17 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 20482-1-AP

Synonyme

TBC1 domain family member 17, TBC1 domain family, member 17, TBC1D17

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  WB analysis using 20482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20482-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 20482-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20482-1-AP (TBC1D17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 20482-1-AP

  IP result of anti-TBC1D17 (IP:20482-1-AP, 4ug; Detection:20482-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human breast cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20482-1-AP (TBC1D17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20482-1-AP (TBC1D17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20482-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20482-1-AP (TBC1D17 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 20482-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20482-1-AP (TBC1D17 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Jurkat-Zellen, Maus-Thymusgewebe, Rattenhirngewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Herzgewebe, humanes Leberkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

20482-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TBC1D17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBC1D17 fusion protein Ag14358
• Vollständiger Name: TBC1 domain family, member 17
• Berechnetes Molekulargewicht: 648 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC003516
• Gene symbol: TBC1D17
• Gene ID (NCBI): 79735
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human		Sci Bull (Beijing); Hepatitis B virus core protein as a Rab-GAP suppressor driving liver disease progression; Authors - Yu Su
human	WB,IF	Cell Death Dis; SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia; Authors - Alice Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HeLa cells