TBC1D22A Polyklonaler Antikörper

TBC1D22A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18332-1-AP

Synonyme

TBC1 domain family, member 22A, TBC1 domain family member 22A, HSC79E021, C22orf4

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  WB analysis of HeLa using 18332-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18332-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18332-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18332-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18332-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18332-1-AP (TBC1D22A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18332-1-AP (TBC1D22A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervix using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervix using 18332-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 18332-1-AP (TBC1D22A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 18332-1-AP

  Immunofluorescent analysis of HeLa cells using 18332-1-AP (TBC1D22A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Zervixgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

18332-1-AP bindet in WB, IHC, IF/ICC, ELISA TBC1D22A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBC1D22A fusion protein Ag12942
• Vollständiger Name: TBC1 domain family, member 22A
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC001292
• Gene symbol: TBC1D22A
• Gene ID (NCBI): 25771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Int J Med Sci; High Expression of TBC1 Domain Family Member 22A is Related to Poor Prognosis in Ovarian Serous Cystadenocarcinoma; Authors - Xiaofeng Lv