TBC1D25 Monoklonaler Antikörper

TBC1D25 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C7H3

Kat-Nr. : 67574-1-PBS

Synonyme

2C7H3, TBC1 domain family member 25, TBC1 domain family, member 25

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  WB analysis using 67574-1-Ig (same clone as 67574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67574-1-Ig (same clone as 67574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67574-1-Ig (same clone as 67574-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 67574-1-Ig (same clone as 67574-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67574-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67574-1-PBS bindet in WB, IF/ICC, Indirect ELISA TBC1D25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TBC1D25 fusion protein Ag29470
• Vollständiger Name: TBC1 domain family, member 25
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC101817
• Gene symbol: TBC1D25
• Gene ID (NCBI): 4943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.