TBC1D4 Polyklonaler Antikörper

TBC1D4 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20024-1-PBS

Synonyme

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  WB analysis using 20024-1-AP (same clone as 20024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20024-1-AP (TBC1D4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 20024-1-AP (same clone as 20024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 20024-1-AP (same clone as 20024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 20024-1-AP (same clone as 20024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20024-1-AP (same clone as 20024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20024-1-AP (same clone as 20024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (20024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 20024-1-AP (same clone as 20024-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 20024-1-AP (same clone as 20024-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20024-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20024-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA TBC1D4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TBC1 domain family, member 4
• Berechnetes Molekulargewicht: 147 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: NM_014832
• Gene symbol: TBC1D4
• Gene ID (NCBI): 9882
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.