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TBC1D5 Rekombinanter Antikörper

TBC1D5 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242534B8

Kat-Nr. : 84990-4-PBS

Synonyme

242534B8, KIAA0210, TBC1 domain family member 5, TBC1 domain family, member 5

Filter:
  • Western Blot (WB) analysis of various lysates using TBC1D5 Recombinant antibody (84990-4-RR)

    WB analysis using 84990-4-RR (same clone as 84990-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84990-4-RR (TBC1D5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84990-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TBC1D5 Recombinant antibody (84990-4-RR)

    IF Staining of A431 using 84990-4-RR (same clone as 84990-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBC1D5 antibody (84990-4-RR, Clone: 242534B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84990-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84990-4-RR

    Affinity and Kinetic Characterization of 84990-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84990-4-RR against Human TBC1D5 were performed. The affinity constant is 3.28 nM.

Geprüfte Anwendungen

Produktinformation

84990-4-PBS bindet in WB, IF/ICC, Indirect ELISA TBC1D5 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TBC1D5 fusion protein Ag10991
Vollständiger Name TBC1 domain family, member 5
Berechnetes Molekulargewicht 795 aa, 89 kDa
Beobachtetes Molekulargewicht 89 kDa
GenBank-ZugangsnummerBC013145
Gene symbol TBC1D5
Gene ID (NCBI) 9779
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBC1D5, a member of TBC (Tre2/Bub2/Cdc16)1 domain family, is a novel retromer-interacting protein. TBC1D5 is a Rab GTPase-activating proteins (GAPs) proteins that negatively regulates VPS35/29/26 recruitment and causes Rab7 to dissociate from the membrane. TBC1D5 could bridge the endosome and autophagosome via its C-terminal LIR motif, and Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy. The TBC1D5 protein exists 89 kDa and 91 kDa isoforms.