TBC1D8 Polyklonaler Antikörper

TBC1D8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 33583-1-AP

Synonyme

AD 3, TBC1 domain family member 8, Vascular Rab-GAP/TBC-containing protein, VRP

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  WB analysis using 33583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33583-1-AP (TBC1D8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 33583-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 33583-1-AP (TBC1D8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Calu-1 cells
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

33583-1-AP bindet in WB, IHC, ELISA TBC1D8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBC1D8 fusion protein Ag35398
• Vollständiger Name: TBC1 domain family, member 8 (with GRAM domain)
• Berechnetes Molekulargewicht: 130kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_001102426
• Gene symbol: TBC1D8
• Gene ID (NCBI): 11138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TBC1D8 protein is a member of the TBC domain family and functions as a GTPase-activating protein (GAP) that negatively regulates the activity of small G proteins of the Rab family, participating in the fine-tuning of intracellular vesicle transport and signal transduction. This protein has garnered significant attention in reproductive and cancer research. Studies have shown that TBC1D8 is a key determinant of oocyte maturation and female fertility, with defects in its expression leading to embryonic development arrest. Moreover, TBC1D8 is abnormally overexpressed in various cancers (such as breast and lung cancer), where it acts as an oncogene by modulating signaling pathways related to cell proliferation, invasion, and metastasis (e.g., the EGFR/Ras pathway). Thus, it serves as a potential biomarker for disease diagnosis and a therapeutic target.