TBCD Polyklonaler Antikörper

TBCD Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14867-1-AP

Synonyme

Beta tubulin cofactor D, KIAA0988, SSD 1, SSD1, TBCD, tfcD, tubulin folding cofactor D, Tubulin specific chaperone D

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Galerie der Validierungsdaten

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  WB analysis using 14867-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14867-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14867-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 14867-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14867-1-AP (TBCD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14867-1-AP

  IP Result of anti-TBCD (IP:14867-1-AP, 4ug; Detection:14867-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

14867-1-AP bindet in WB, IP, IF, ELISA TBCD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBCD fusion protein Ag6310
• Vollständiger Name: tubulin folding cofactor D
• Berechnetes Molekulargewicht: 133 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC003094
• Gene symbol: TBCD
• Gene ID (NCBI): 6904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	ACS Chem Neurosci; Proteomic Study on the Mechanism of Arsenic Neurotoxicity in the Rat Cerebral Cortex and the Protective Mechanism of Dictyophora Polysaccharides against Arsenic Neurotoxicity; Authors - Xinglai Zhang
human	IF	Genes Chromosomes Cancer; MYCN overexpression is associated with unbalanced copy number gain, altered nuclear location, and overexpression of chromosome arm 17q genes in neuroblastoma tumors and cell lines.; Authors - Alexandra Kuzyk
mouse	IF	Histochem Cell Biol; Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.; Authors - Lukas Juergens
human	WB	Front Genet; Target-Sequencing of Female Infertility Pathogenic Gene Panel and a Novel TUBB8 Loss-of-Function Mutation.; Authors - Hongxia Yuan