TBCD Rekombinanter Antikörper

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• KD/KO Validated

TBCD Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230209H7

Kat-Nr. : 82911-1-RR

Synonyme

SSD-1, SSD1, PP1096, KIAA0988, Beta-tubulin cofactor D

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  WB analysis of MCF-7 using 82911-1-RR

  WB result of TBCD antibody (82911-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBCD transfected MCF-7 cells.

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  WB analysis using 82911-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82911-1-RR (TBCD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 82911-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TBCD antibody (82911-1-RR, Clone: 230209H7 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

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  FC experiment of MCF-7 using 82911-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TBCD Recombinant antibody (82911-1-RR, Clone:230209H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82911-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82911-1 -RR against Human TBCD were performed. The affinity constant is 1.52 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82911-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA TBCD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TBCD fusion protein Ag6310
• Vollständiger Name: tubulin folding cofactor D
• Berechnetes Molekulargewicht: 133 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC003094
• Gene symbol: TBCD
• Gene ID (NCBI): 6904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.