TBK1 Monoklonaler Antikörper

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• KD/KO Validated

TBK1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Ratte und mehr (2)

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(27)

CloneNo.

2D7B1

Kat-Nr. : 67211-1-Ig

Synonyme

Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1

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  WB analysis using 67211-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 67211-1-Ig

  U2OS (WT and TBK1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 67211-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, LNCaP-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

67211-1-Ig bindet in WB, IF, IP, ELISA TBK1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TBK1 fusion protein Ag28894
• Vollständiger Name: TANK-binding kinase 1
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC034950
• Gene symbol: TBK1
• Gene ID (NCBI): 29110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.

Publikationen

Species	Application	Title
mouse	WB	J Med Chem; Development of Orally Bioavailable Amidobenzimidazole Analogues Targeting Stimulator of Interferon Gene (STING) Receptor; Authors - Nan-Nan Chen
human	WB	J Virol; Porcine deltacoronavirus nsp5 antagonizes type I interferon signaling by cleaving IFIT3; Authors - Haixin Huang
human	WB	Front Immunol; Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.; Authors - Zhihai Zhou
pig	WB	Vet Res; ASFV pS183L protein negatively regulates RLR-mediated antiviral signalling by blocking MDA5 oligomerisation; Authors - Huan Chen
human	WB	Heliyon; Metformin promotes cGAS/STING signaling pathway activation by blocking AKT phosphorylation in gastric cancer; Authors - Qian Shen
human	WB	Biomed Pharmacother; IFI16 promotes cervical cancer progression by upregulating PD-L1 in immunomicroenvironment through STING-TBK1-NF-kB pathway.; Authors - Hongning Cai