TBK1 Rekombinanter Antikörper

TBK1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240769G5

Kat-Nr. : 83686-3-PBS

Synonyme

240769G5, EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1

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  WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 83686-3-RR (same clone as 83686-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83686-3-RR (same clone as 83686-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83686-3-RR (same clone as 83686-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TBK1 antibody (83686-3-RR, Clone: 240769G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83686-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83686-3-PBS bindet in WB, IHC, IF/ICC, ELISA TBK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TBK1 fusion protein Ag28894
• Vollständiger Name: TANK-binding kinase 1
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC034950
• Gene symbol: TBK1
• Gene ID (NCBI): 29110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.