TBK1 Rekombinanter Antikörper

TBK1 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240769G5

Kat-Nr. : 83686-3-RR

Synonyme

240769G5, EC:2.7.11.1, NF kappa B activating kinase, NF-kappa-B-activating kinase, Serine/threonine protein kinase TBK1

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  WB analysis using 83686-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83686-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83686-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83686-3-RR (TBK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83686-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83686-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 83686-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 83686-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83686-3-RR (TBK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 83686-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TBK1 antibody (83686-3-RR, Clone: 240769G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 4T1-Zellen, C6-Zellen, HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausnierengewebe, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83686-3-RR bindet in WB, IHC, IF/ICC, ELISA TBK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TBK1 fusion protein Ag28894
• Vollständiger Name: TANK-binding kinase 1
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC034950
• Gene symbol: TBK1
• Gene ID (NCBI): 29110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.