TBP Polyklonaler Antikörper

TBP Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22246-1-AP

Synonyme

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

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  WB analysis of Jurkat using 22246-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 22246-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 22246-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22246-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 22246-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 22246-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TBP antibody 22246-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, COLO 320-Zellen, HeLa-Zellen, Maus-Kolongewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

22246-1-AP bindet in WB, IF/ICC, ELISA TBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBP fusion protein Ag17740
• Vollständiger Name: TATA box binding protein
• Berechnetes Molekulargewicht: 338 aa, 38 kDa
• Beobachtetes Molekulargewicht: mouse/rat 33-36 kDa and human 37-43kDa
• GenBank-Zugangsnummer: BC110341
• Gene symbol: TBP
• Gene ID (NCBI): 6908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This is a rabbit polyclonal antibody raised against part of N-terminal chain of human TBP, and specially recognize a ~46 kDa band.

Publikationen

Species	Application	Title
mouse	WB	Breast Cancer; Isoforms of IDH in breast carcinoma: IDH2 as a potent prognostic factor associated with proliferation in estrogen-receptor positive cases.; Authors - Hiroyuki Minemura
human	WB	Mol Med Rep; Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment.; Authors - Qingye Li