TBP Monoklonaler Antikörper

TBP Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H3B2

Kat-Nr. : CL488-66166

Synonyme

TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1

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  IF Staining of C2C12 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CL488-66166 (TBP antibody) at dilution of 1:50.

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  IF Staining of A431 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TBP antibody (CL488-66166, Clone: 2H3B2 ) at dilution of 1:100.

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  IF Staining of C2C12 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 488-conjugated TBP antibody (CL488-66166, Clone: 2H3B2 ) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66166

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human TBP (CL488-66166, Clone:2H3B2) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66166 bindet in IF/ICC, FC (Intra) TBP und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TBP fusion protein Ag12383
• Vollständiger Name: TATA box binding protein
• Berechnetes Molekulargewicht: 338 aa, 38 kDa
• GenBank-Zugangsnummer: BC110341
• Gene symbol: TBP
• Gene ID (NCBI): 6908
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).