TBPL1 Polyklonaler Antikörper

TBPL1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 12258-1-AP

Synonyme

21 kDa TBP like protein, STUD, TBP like 1, TBP like factor, TBP like protein 1, TBP related factor 2, TBP related protein, TBPL1, TLF, TLP, TLP21, TRF2, TRP

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12258-1-AP (TBPL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12258-1-AP

  IP result of anti-TBPL1 (IP:12258-1-AP, 4ug; Detection:12258-1-AP 1:300) with mouse testis tissue lysate 3920 ug.

• 

  IHC staining of human stomach cancer using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12258-1-AP (TBPL1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, Maushodengewebe, Maus-Magengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

12258-1-AP bindet in WB, IP, IHC, IF, ELISA TBPL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBPL1 fusion protein Ag2880
• Vollständiger Name: TBP-like 1
• Berechnetes Molekulargewicht: 186 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa, 25 kDa
• GenBank-Zugangsnummer: BC017559
• Gene symbol: TBPL1
• Gene ID (NCBI): 9519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell; A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.; Authors - Peng Dai
mouse	WB	Pharmacol Res; Fluvoxamine alleviates bleomycin-induced lung fibrosis via regulating the cGAS-STING pathway; Authors - Xiaohua Xie
human	WB	Cell Signal; C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1.; Authors - Hui Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jianing (Verified Customer) (09-03-2019)

This antibody recognize human tbpl1 strongly which give a nice clear band. But the efficiency against zebrafish tbpl1 is very low.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cell culture lysate