TBR1 Monoklonaler Antikörper

TBR1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

5C3A5

Kat-Nr. : 66564-1-Ig

Synonyme

T box brain protein 1, T box, brain, 1, T brain 1, T-box brain protein 1, TBR 1

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  WB analysis using 66564-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of pig cerebellum using 66564-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of rat cerebellum using 66564-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66564-1-Ig (TBR1 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IHC staining of mouse brain using 66564-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66564-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66564-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66564-1-Ig (TBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 66564-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 66564-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TBR1 antibody (66564-1-Ig, Clone: 5C3A5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, Maushirngewebe, Hausschwein-Cerebellum-Gewebe, Kaninchenhirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 4 publications below
• IF: See 5 publications below

Produktinformation

66564-1-Ig bindet in WB, IHC, IF-P, ELISA TBR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TBR1 fusion protein Ag15262
• Vollständiger Name: T-box, brain, 1
• Berechnetes Molekulargewicht: 682 aa, 74 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC104844
• Gene symbol: TBR1
• Gene ID (NCBI): 10716
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBR1, also named T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.; Authors - Huai-Bin Hu
mouse	IHC	EMBO J; Centrosome defects cause microcephaly by activating the 53BP1-USP28-TP53 mitotic surveillance pathway.; Authors - Thao P Phan
mouse	IF	Ann Neurol; Pathogenic MAST3 Variants in the STK Domain Are Associated with Epilepsy.; Authors - Egidio Spinelli
mouse	IF	Biochem Biophys Res Commun; Neuron-specific loss of Ppp6c induces neonatal death and decreases the number of cortical neurons and interneurons; Authors - Miki Matsuoka
human	IHC	Nat Commun; iPSC-derived models of PACS1 syndrome reveal transcriptional and functional deficits in neuron activity; Authors - Lauren Rylaarsdam
rat	IF	J Hazard Mater; Effects of nanoplastic exposure during pregnancy and lactation on neurodevelopment of rat offspring; Authors - Lei Tian