TBRG4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TBRG4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 16245-1-AP

Synonyme

CPR2, FASTKD4, KIAA0948, Protein TBRG4, TBRG4

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  WB analysis of HepG2 using 16245-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16245-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16245-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 16245-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16245-1-AP (TBRG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16245-1-AP

  IP result of anti-TBRG4 (IP:16245-1-AP, 4ug; Detection:16245-1-AP 1:500) with HepG2 cells lysate 1600ug.

• 

  IHC staining of human liver cancer using 16245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16245-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16245-1-AP (TBRG4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS cells using 16245-1-AP

  IF result of anti-TBRG4 (16245-1-AP) with U2OS cells.

• 

  IF Staining of HeLa using 16245-1-AP

  Immunofluorescent analysis of Hela cells, using TBRG4 antibody 16245-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of COS7 cells using 16245-1-AP

  IF result of 16245-1-AP (anti-KD4) in COS7 cell by Dr. Nancy Kedersha. COS7 cells, fixed with 4% paraformaldehyde, post/fied and permeabilized with methanol, and stained with PTG anti-FAST KD4 antibody(16245-1-AP) in green, and human anti-mito autoimune sera from Immunovision in red. Nuclei are stained blue using Hoechst dye.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Lebergewebe, Maus-Eierstockgewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : N/A

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16245-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TBRG4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBRG4 fusion protein Ag9176
• Vollständiger Name: transforming growth factor beta regulator 4
• Berechnetes Molekulargewicht: 631 aa, 71 kDa
• Beobachtetes Molekulargewicht: 60 kDa, 71 kDa
• GenBank-Zugangsnummer: BC017235
• Gene symbol: TBRG4
• Gene ID (NCBI): 9238
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBRG4 is also named as CPR2(Cell cycle progression restoration protein 2), FASTKD4(FAST kinase domain-containing protein 4), KIAA0948 and belongs to the FAST kinase family. This protein contains multiple putative leucine zipper domains characteristic of transcription factors and it may play a role in cell cycle progression(PMID:9383053). RT-PCR ELISA detected moderate expression in ovary, and little to no expression in all other tissues and specific brain regions examined(PMID:10231032).TBRG4 has an mature form with MW of 60 kDa produced by transit peptide missing.

Publikationen

Species	Application	Title
human	WB	PLoS Genet; The FASTK family proteins fine-tune mitochondrial RNA processing; Authors - Akira Ohkubo ; KO Validated
human	WB,IHC,IF,CoIP	BMC Cancer; High expression of TBRG4 in relation to unfavorable outcome and cell ferroptosis in hepatocellular carcinoma; Authors - Shanchun Tao
human	WB	Life Sci Alliance; Silica-based solid-phase extraction of cross-linked nucleic acid-bound proteins.; Authors - Claudio Asencio
human	WB,IP	Nucleic Acids Res; The Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA; Authors - Xuan Yang ; KO Validated
human	WB,IP,IF	Spine J; TBRG4 regulates ubiquitination of Beclin1 and participates in autophagy pathway to inhibit intervertebral disc degeneration; Authors - Xilong Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Erik (Verified Customer) (02-24-2021)

The antibody used at 1:500 dilution produces a clean and strong band at the expected height for the protein

• Applications: Western Blot
• Cell Tissue Type: PANC1 and MIAPACA2 cell lines

TBRG4 Antibody Western Blot validation ( dilution) in PANC1 and MIAPACA2 cell lines (Cat no:16245-1-AP)