TBX20 Rekombinanter Antikörper

TBX20 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240385H6

Kat-Nr. : 83414-3-PBS

Synonyme

TBX 20, T-box transcription factor TBX20, T-box protein 20, T box 20, ASD4

---

• 

  IHC staining of mouse heart using 83414-3-RR (same clone as 83414-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83414-3-RR (TBX20 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

• 

  IF Staining of H9C2 using 83414-3-RR (same clone as 83414-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using TBX20 antibody (83414-3-RR, Clone: 240385H6 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

• 

  FC experiment of K562 using 83414-3-RR (same clone as 83414-3-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug Tbx20 Recombinant Antibody (83414-3-RR, Clone:240385H6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83414-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00401-1

  Cytometric bead array standard curve of MP00401-1, TBX20 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83414-3-PBS. Detection antibody: 83414-1-PBS. Standard: Ag18501. Range: 0.625-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 83414-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83414-3-RR against Human TBX20 were performed. The affinity constant is 17 nM.

---

Geprüfte Anwendungen

Produktinformation

83414-3-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA TBX20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TBX20 fusion protein Ag18501
• Vollständiger Name: T-box 20
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• GenBank-Zugangsnummer: BC120946
• Gene symbol: TBX20
• Gene ID (NCBI): 57057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tbx20 is a transcription factor that is essential for proper heart development in a growing fetus. Any mutations in this gene can result in various forms of congenital heart disease.