TBX4/5 Rekombinanter Antikörper

TBX4/5 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242565F1

Kat-Nr. : 84997-4-RR

Synonyme

TBX5, HOS, T box 5, T-box protein 5, T-box transcription factor TBX5

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  IF Staining of U2OS using 84997-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TBX5 antibody (84997-4-RR, Clone: 242565F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84997-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84997-4-RR against Human TBX4/5 were performed. The affinity constant is 37.3 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84997-4-RR bindet in IF/ICC, ELISA TBX4/5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TBX4/5 fusion protein Ag3845
• Vollständiger Name: T-box 5
• Berechnetes Molekulargewicht: 518 aa, 57 kDa
• GenBank-Zugangsnummer: BC027942
• Gene symbol: TBX5
• Gene ID (NCBI): 6910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

T-box genes encode a family of transcription factors that have been identified in all metazoans and which play diverse roles during embryonic development. TBX5, a member of T-box family, has an essential role in the initiation of limb outgrowth and heart development. Genetic variation near TBX5 that associates with PR and/or QRS interval variation, which suggests that TBX5 plays a role in CCS function in the general population. Because 77 % amino acid sequence of TBX5 is identical to TBX4, this antibody recognizes both TBX4 and TBX5.