TBXAS1 Polyklonaler Antikörper

TBXAS1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 11321-1-AP

Synonyme

CYP5, CYP5A1, Cytochrome P450 5A1, GHOSAL, TBXAS1, THAS, Thromboxane A synthase, TS, TXA synthase, TXAS, TXS

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  WB analysis of A549 using 11321-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11321-1-AP (TBXAS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11321-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11321-1-AP (TBXAS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11321-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11321-1-AP (TBXAS1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

11321-1-AP bindet in WB, IHC, IF, ELISA TBXAS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TBXAS1 fusion protein Ag1807
• Vollständiger Name: thromboxane A synthase 1 (platelet)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC014117
• Gene symbol: TBXAS1
• Gene ID (NCBI): 6916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in humen uterus (PMID:1386258).

Publikationen

Species	Application	Title
human	WB,IHC	Hypertension; Reduced methylation of the thromboxane synthase gene is correlated with its increased vascular expression in preeclampsia.; Authors - Mousa Ahmad A AA
human	IF	Respir Res; Clonally expanded human airway smooth muscle cells exhibit morphological and functional heterogeneity.; Authors - Shailendra R Singh
human	IHC,IF	Am J Physiol Lung Cell Mol Physiol; Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways?; Authors - Singh Shailendra R SR
		Am J Physiol Renal Physiol; Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.; Authors - Katharina Mederle