TCERG1 Polyklonaler Antikörper

TCERG1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21858-1-AP

Synonyme

CA150, TAF2S, TCERG1, Transcription factor CA150

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  WB analysis using 21858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21858-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21858-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21858-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21858-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21858-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21858-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21858-1-AP bindet in WB, IHC, IF/ICC, ELISA TCERG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCERG1 fusion protein Ag16482
• Vollständiger Name: transcription elongation regulator 1
• Berechnetes Molekulargewicht: 1098 aa, 124 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC111727
• Gene symbol: TCERG1
• Gene ID (NCBI): 10915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.