TCF12 Monoklonaler Antikörper

TCF12 Monoklonal Antikörper für Indirect ELISA, WB

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

Indirect ELISA, WB

Konjugation

Unkonjugiert

CloneNo.

1B8D4

Kat-Nr. : 67523-1-PBS

Synonyme

TCF12/HEB, E-box-binding protein, E box binding protein, DNA-binding protein HTF4, Class B basic helix-loop-helix protein 20

---

• 

  WB analysis using 67523-1-Ig (same clone as 67523-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67523-1-Ig (same clone as 67523-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67523-1-Ig (same clone as 67523-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67523-1-Ig (same clone as 67523-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67523-1-Ig (TCF12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 67523-1-Ig (same clone as 67523-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human TCF12 (67523-1-Ig, Clone:1B8D4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67523-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67523-1-PBS bindet in Indirect ELISA, WB TCF12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TCF12 fusion protein Ag6287
• Vollständiger Name: transcription factor 12
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC050556
• Gene symbol: TCF12
• Gene ID (NCBI): 6938
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].