TCF12 Monoklonaler Antikörper

TCF12 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B8D4

Kat-Nr. : CL488-67523

Synonyme

TCF12, TCF12/HEB, transcription factor 12, Transcription factor HTF-4, TCF-12, 1B8D4, bHLHb20, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E box binding protein, E-box-binding protein, HEB, HsT17266, HTF4, TCF 12

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  FC experiment of HepG2 using CL488-67523

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TCF12 (CL488-67523, Clone:1B8D4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67523 bindet in FC (Intra) TCF12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TCF12 fusion protein Ag6287
• Vollständiger Name: transcription factor 12
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC050556
• Gene symbol: TCF12
• Gene ID (NCBI): 6938
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].