TCF3 Polyklonaler Antikörper

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• KD/KO Validated

TCF3 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 21242-1-AP

Synonyme

E2A, ITF1, Immunoglobulin enhancer-binding factor E12/E47, E47, Class B basic helix-loop-helix protein 21

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  WB analysis of Jurkat using 21242-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21242-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 21242-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 21242-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 21242-1-AP

  IP result of anti-TCF3 (IP:21242-1-AP, 4ug; Detection:21242-1-AP 1:500) with Jurkat cells lysate 2800ug.

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  IF Staining of HeLa using 21242-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TCF3 antibody (21242-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

21242-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA TCF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCF3 fusion protein Ag15734
• Vollständiger Name: transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Berechnetes Molekulargewicht: 654 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC110580
• Gene symbol: TCF3
• Gene ID (NCBI): 6929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Nat Cell Biol; Mapping putative enhancers in mouse oocytes and early embryos reveals TCF3/12 as key folliculogenesis regulators; Authors - Bofeng Liu
human	WB,CoIP	Cell Signal; Targeting SDCBP2 in acute myeloid leukemia; Authors - Yan Du
human	WB	J Cell Mol Med; The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.; Authors - Meiju Zhang ; KD Validated
mouse	WB	Mol Immunol; Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development.; Authors - Wei Liang
rat	IP	Life Sci; miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling.; Authors - Ting Liu
mouse	WB	Nat Commun; A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation; Authors - Jianfeng Wu