TCF4 Polyklonaler Antikörper

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• KD/KO Validated

TCF4 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(66)

Kat-Nr. : 22337-1-AP

Synonyme

ITF-2, ITF2, ITF 2, E2 2, Class B basic helix-loop-helix protein 19

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  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22337-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 22337-1-AP

  IP result of anti-TCF4 (IP:22337-1-AP, 4ug; Detection:22337-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse brain using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22337-1-AP (TCF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22337-1-AP (TCF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:200.

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  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:200.

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  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 22337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4 Antibody) at dilution of 1:50.

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  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 22337-1-AP (TCF4 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TCF4 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TCF4 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 22337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 22337-1-AP (TCF4 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, K-562-Zellen
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 55 publications below
• IHC: See 8 publications below
• IF: See 11 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below
• ChIP: See 3 publications below

Produktinformation

22337-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA TCF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, drosophila, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCF4 fusion protein Ag17813
• Vollständiger Name: transcription factor 4
• Berechnetes Molekulargewicht: 671 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC125084
• Gene symbol: TCF4
• Gene ID (NCBI): 6925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor 4 (TCF4), also known as SEF2, ITF2, E2-2, and ME2, binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Human TCF4 mRNA expression is particularly high in the brain. Usage of numerous 5' exons of the human TCF4 gene potentially yields in TCF4 protein isoforms with 18 different N-termini. In addition, the diversity of isoforms is increased by alternative splicing of several internal exons. Some isoforms contain a bipartite nuclear localization signal and are exclusively nuclear, whereas distribution of other isoforms relies on heterodimerization partners. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression(15547706, 12796377). The scopel of molecular weight of several isoforms is about 60-90 kDa (21789225).

Publikationen

Species	Application	Title
	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Adv Sci (Weinh); Nuclear to Cytoplasmic Transport Is a Druggable Dependency in HDAC7-driven Small Cell Lung Cancer; Authors - Tingting Qin
	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Nat Commun; Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes.; Authors - Norman L Lehman
	WB,IF,ChIP	Acta Pharmacol Sin; Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction; Authors - Jing Li
human	IHC	JCI Insight; Suppression of TCF4 promotes a ZC3H12A-mediated self-sustaining inflammatory feedback cycle involving IL-17RA/IL-17RE epidermal signaling; Authors - Yanyun Jiang