TCF1/TCF7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TCF1/TCF7 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Kat-Nr. : 14464-1-AP

Synonyme

TCF7, TCF-1, TCF1, TCF 7, TCF 1

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  WB analysis of mouse thymus using 14464-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14464-1-AP (TCF7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 14464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TCF1/TCF7 antibody (14464-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 5 publications below
• IP: See 1 publications below

Produktinformation

14464-1-AP bindet in WB, IHC, IF-P, IP, ELISA TCF1/TCF7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCF1/TCF7 fusion protein Ag5792
• Vollständiger Name: transcription factor 7 (T-cell specific, HMG-box)
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC048769
• Gene symbol: TCF1/TCF7
• Gene ID (NCBI): 6932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
human	WB,IHC	Clin Cancer Res; Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.; Authors - Huanjun Wang ; KD Validated
human,mouse	WB	Int J Biol Macromol; Correlation between TCF7 and bladder cancer and feasibility of Erlotinib targeting in bladder cancer: Molecular mechanism and expression of TCF7 recombinant protein; Authors - Zengmi Mo
human	WB	Clin Transl Med; Long noncoding RNA LINC01606 protects colon cancer cells from ferroptotic cell death and promotes stemness by SCD1-Wnt/β-catenin-TFE3 feedback loop signalling.; Authors - Yajun Luo
mouse	WB	iScience; Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity; Authors - Ting He
human	WB	J Transl Med; Forkhead Box S1 mediates epithelial-mesenchymal transition through the Wnt/β-catenin signaling pathway to regulate colorectal cancer progression.; Authors - Liang Zhang