TCF7L1 Polyklonaler Antikörper

TCF7L1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14519-1-AP

Synonyme

HMG box transcription factor 3, TCF 3, TCF3, TCF7L1, Transcription factor 7 like 1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14519-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14519-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14519-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 14519-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, JAR-Zellen, Maushirngewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

14519-1-AP bindet in WB, IHC, IF, ELISA TCF7L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCF7L1 fusion protein Ag5983
• Vollständiger Name: transcription factor 7-like 1 (T-cell specific, HMG-box)
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC058894
• Gene symbol: TCF7L1
• Gene ID (NCBI): 83439
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
zebrafish	IF	Nat Commun; Wnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling.; Authors - Kathleen Hübner
human	IF	Stem Cell Reports; Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta.; Authors - Sandra Haider
human	IHC	Oncogenesis; TCF7L1 regulates cytokine response and neuroendocrine differentiation of prostate cancer.; Authors - Yu-Ching Wen
mouse	WB	iScience; Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity; Authors - Ting He
human	WB,IF	Endocrinology; Wnt-dependent T-cell factor-4 controls human extravillous trophoblast motility.; Authors - Gudrun Meinhardt