TCF4/TCF7L2 Polyklonaler Antikörper

TCF4/TCF7L2 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 13838-1-AP

Synonyme

HMG box transcription factor 4, hTCF 4, T cell factor 4, TCF 4, TCF4, TCF4/TCF7L2, TCF7L2, Transcription factor 7 like 2

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Galerie der Validierungsdaten

• 

  WB analysis using 13838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF4/TCF7L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13838-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13838-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 13838-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13838-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13838-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13838-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13838-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13838-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13838-1-AP (TCF7L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13838-1-AP

  IP Result of anti-TCF7L2 (IP:13838-1-AP, 4ug; Detection:13838-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, Mausherzgewebe, Mausnierengewebe, Maus-Plazenta-Gewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 19 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

13838-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA TCF4/TCF7L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCF4/TCF7L2 fusion protein Ag4786
• Vollständiger Name: transcription factor 7-like 2 (T-cell specific, HMG-box)
• Berechnetes Molekulargewicht: 619 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC032656
• Gene symbol: TCF7L2
• Gene ID (NCBI): 6934
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
human	WB	Oncogene; The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.; Authors - Kai Li
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
human	WB,IF,CoIP	Front Cell Dev Biol; Twist1 Contributes to the Maintenance of Some Biological Properties of Dermal Papilla Cells in vitro by Forming a Complex With Tcf4 and β-Catenin.; Authors - Nanlan Yu
human	WB	Mol Cancer Res; Wnt/β-Catenin Signaling Axis Is Required for TFEB-Mediated Gastric Cancer Metastasis and Epithelial-Mesenchymal Transition.; Authors - Shuxuan Li
human	WB	J Biol Chem; HBP1-mediated regulation of p21 through Mdm2/p53 and TCF4/EZH2 pathways and its impact on cell senescence and tumorigenesis.; Authors - Yifan Chen