TCIRG1 Polyklonaler Antikörper

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TCIRG1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12649-1-PBS

Synonyme

T-cell immune regulator 1, OPTB1, OC-116 kDa, OC116, ATP6V0A3

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  WB analysis using 12649-1-AP (same clone as 12649-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12649-1-AP (TCIRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 12649-1-AP (same clone as 12649-1-PBS)

  WB result of TCIRG1 antibody (12649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCIRG1 transfected A431 cells. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 12649-1-AP (same clone as 12649-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 12649-1-AP (TCIRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12649-1-AP (same clone as 12649-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12649-1-AP (TCIRG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12649-1-AP (same clone as 12649-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using TCIRG1 antibody 12649-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12649-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TCIRG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCIRG1 fusion protein Ag3378
• Vollständiger Name: T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
• Berechnetes Molekulargewicht: 830 aa, 93 kDa
• Beobachtetes Molekulargewicht: 92 kDa, 65-70 kDa
• GenBank-Zugangsnummer: BC018133
• Gene symbol: TCIRG1
• Gene ID (NCBI): 10312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCIRG1, also known as V ATPase 116 kDa isoform a3, TIRC7, OPTB1, belongs to the V-ATPase 116 kDa subunit family, TCIRG1 seems to be directly involved in T-cell activation (PubMed:10329006). Alternative splicing results in multiple transcript variants. Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus (PMID: 15809087, 24753205). Mutations in TCIRG1 are associated with Osteopetrosis, autosomal recessive 1 (OPTB1)( PMID: 11532986).