TCIRG1 Rekombinanter Antikörper

TCIRG1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240166B2

Kat-Nr. : 83351-6-RR

Synonyme

a3, Atp6i, ATP6N1C, ATP6V0A3, OC 116 kDa, OC 116kDa, OC116, OPTB1, Stv1, T cell immune regulator 1, TCIRG1, TIRC7, V ATPase 116 kDa isoform a3, Vph1

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  WB analysis using 83351-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83351-6-RR (TCIRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83351-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83351-6-RR against Human TCIRG1 were performed. The affinity constant is 2.14 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, Daudi-Zellen, HepG2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83351-6-RR bindet in WB, ELISA TCIRG1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TCIRG1 fusion protein Ag3378
• Vollständiger Name: T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
• Berechnetes Molekulargewicht: 830 aa, 93 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC018133
• Gene symbol: TCIRG1
• Gene ID (NCBI): 10312
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCIRG1, also known as V ATPase 116 kDa isoform a3, TIRC7, OPTB1, belongs to the V-ATPase 116 kDa subunit family, TCIRG1 seems to be directly involved in T-cell activation (PubMed:10329006). Alternative splicing results in multiple transcript variants. Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus (PMID: 15809087, 24753205). Mutations in TCIRG1 are associated with Osteopetrosis, autosomal recessive 1 (OPTB1)( PMID: 11532986).