TCL1A Rekombinanter Antikörper

TCL1A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242623A9

Kat-Nr. : 85129-5-RR

Synonyme

242623A9, Oncogene TCL-1, T-cell leukemia/lymphoma protein 1A, TCL1

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  WB analysis of Ramos using 85129-5-RR

  Ramos cells were subjected to SDS PAGE followed by western blot with 85129-5-RR (TCL1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 85129-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85129-5-RR (TCL1A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Ramos using 85129-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using TCL1A antibody (85129-5-RR, Clone: 242623A9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Ramos using 85129-5-RR

  1x10^6 Ramos cells were intracellularly stained with 0.25 ug TCL1A Recombinant antibody (85129-5-RR, Clone:242623A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 85129-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85129-5-RR against Human TCL1A were performed. The affinity constant is 0.185 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Ramos-Zellen
• Erfolgreiche Detektion in FC (Intra): Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85129-5-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA TCL1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TCL1A fusion protein Ag0786
• Vollständiger Name: T-cell leukemia/lymphoma 1A
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC005831
• Gene symbol: TCL1A
• Gene ID (NCBI): 8115
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCL1A, also named as TCL1 and p14 TCL1, enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. TCL1A immunodetection is an independent marker of adverse outcome that could be used in routine settings for the management of DLCL patients. TCL1A expression is correlated with shorter time to treatment in chronic lymphocytic leukemia cases and shorter lymphoma-specific survival in mantle cell lymphoma series. It is a potential therapeutic target.