TCN2 Polyklonaler Antikörper

TCN2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 12157-1-AP

Synonyme

D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

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Galerie der Validierungsdaten

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  WB analysis using 12157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 12157-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12157-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse small intestine using 12157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 12157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12157-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, Maus-Dünndarmgewebe, MCF-7-Zellen, Rattenlebergewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

12157-1-AP bindet in WB, IHC, IF, ELISA TCN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCN2 fusion protein Ag2825
• Vollständiger Name: transcobalamin II; macrocytic anemia
• Berechnetes Molekulargewicht: 400 aa, 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC011239
• Gene symbol: TCN2
• Gene ID (NCBI): 6948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

Publikationen

Species	Application	Title
canine, cat	IHC	Oncotarget; Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.; Authors - Annette M Sysel
mouse	IF	Int J Biol Macromol; CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.; Authors - Yue Chen
human	IHC	Anticancer Res; Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.; Authors - Sysel Annette M AM
rat	WB	Mol Pharm; Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin; Authors - Yuri Kinoshita

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joseph (Verified Customer) (09-17-2020)

We evaluated several antibodies against TCN2 and found the Proteintech sequence to provide the best sensitivity and specificity compared to the other companies

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline
• Cell Tissue Type: human, canine and feline malignant various