TCN2 Polyklonaler Antikörper

TCN2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12157-1-PBS

Synonyme

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  WB analysis using 12157-1-AP (same clone as 12157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 12157-1-AP (same clone as 12157-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 12157-1-AP (same clone as 12157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12157-1-AP (same clone as 12157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12157-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12157-1-PBS bindet in WB, IHC, Indirect ELISA TCN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCN2 fusion protein Ag2825
• Vollständiger Name: transcobalamin II; macrocytic anemia
• Berechnetes Molekulargewicht: 400 aa, 44 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC011239
• Gene symbol: TCN2
• Gene ID (NCBI): 6948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)