TCOF1 Polyklonaler Antikörper

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• KD/KO Validated

TCOF1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 11003-1-AP

Synonyme

treacle, MFD1

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  WB analysis of Jurkat using 11003-1-AP

  WB result of TCOF1 antibody (11003-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCOF1 transfected Jurkat cells.

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  WB analysis of HeLa using 11003-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 11003-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11003-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11003-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11003-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody 11003-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MCF-7 using 11003-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (11003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

11003-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA TCOF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCOF1 fusion protein Ag1447
• Vollständiger Name: Treacher Collins-Franceschetti syndrome 1
• Berechnetes Molekulargewicht: 97 kDa, 144 kDa, 152 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: BC014559
• Gene symbol: TCOF1
• Gene ID (NCBI): 6949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

Publikationen

Species	Application	Title
human	WB	Nature; Cell-fate determination by ubiquitin-dependent regulation of translation.; Authors - Achim Werner
human		Nat Commun; Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.; Authors - Stephen M Rawlinson
human	WB, IF	Proc Natl Acad Sci U S A; Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.; Authors - Alberto Ciccia ; KD Validated
human	WB	Elife; Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.; Authors - Achim Werner
human	WB, IF	Int J Mol Sci; TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.; Authors - Ahmed Hassan
human	WB,IF	Cancer Sci; TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.; Authors - Chaoshen Wu