TCP1 Polyklonaler Antikörper

TCP1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10320-1-AP

Synonyme

CCT alpha, CCT1, CCTa, D6S230E, t complex 1, TCP 1 alpha, TCP1

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Galerie der Validierungsdaten

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  WB analysis using 10320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10320-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10320-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10320-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10320-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10320-1-AP

  IP Result of anti-TCP1 (IP:10320-1-AP, 4ug; Detection:10320-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 10320-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TCP1 antibody (10320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10320-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10320-1-AP (TCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10320-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TCP1 antibody (10320-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10320-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TCP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCP1 fusion protein Ag0389
• Vollständiger Name: t-complex 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC000665
• Gene symbol: TCP1
• Gene ID (NCBI): 6950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
mouse,human	WB	Front Cell Dev Biol; TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis; Authors - Wen-Shuai Tang
mouse	WB	J Alzheimers Dis; Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid; Authors - Ye Shan
mouse	IP	Virology; Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.; Authors - Sandhya Gopal
mouse	WB	J Agric Food Chem; A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.; Authors - Cheng Liu