TCP1 Polyklonaler Antikörper

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• KD/KO Validated

TCP1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 10320-1-AP

Synonyme

CCT alpha, CCT1, CCTa, CCT-alpha, Chaperonin containing T-complex polypeptide 1 subunit 1

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  WB analysis using 10320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10320-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10320-1-AP

  IP result of anti-TCP1 (IP:10320-1-AP, 4ug; Detection:10320-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 10320-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TCP1 antibody (10320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10320-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10320-1-AP (TCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10320-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TCP1 Polyclonal antibody (10320-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Mausherzgewebe, NCI-H1299-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:800-1:3200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

10320-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA TCP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCP1 fusion protein Ag0389
• Vollständiger Name: t-complex 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC000665
• Gene symbol: TCP1
• Gene ID (NCBI): 6950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

0

Publikationen

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
mouse,human	WB	Front Cell Dev Biol; TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis; Authors - Wen-Shuai Tang
mouse	WB	J Alzheimers Dis; Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid; Authors - Ye Shan
mouse	IP	Virology; Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.; Authors - Sandhya Gopal

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