TCP1 Monoklonaler Antikörper

TCP1 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C2F6

Kat-Nr. : 68183-1-PBS

Synonyme

D6S230E, Chaperonin containing T-complex polypeptide 1 subunit 1, CCT-alpha, CCTa, CCT1

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  WB analysis using 68183-1-Ig (same clone as 68183-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68183-1-Ig (TCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TCP1 antibody (68183-1-Ig, Clone: 2C2F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TCP1 (68183-1-Ig, Clone:2C2F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68183-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA TCP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TCP1 fusion protein Ag0389
• Vollständiger Name: t-complex 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC000665
• Gene symbol: TCP1
• Gene ID (NCBI): 6950
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.