TCP11L2 Rekombinanter Antikörper

TCP11L2 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240096F6

Kat-Nr. : 83227-1-PBS

Synonyme

T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6

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  WB analysis using 83227-1-RR (same clone as 83227-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 83227-1-RR (same clone as 83227-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83227-1-RR (same clone as 83227-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TCP11L2 antibody (83227-1-RR, Clone: 240096F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83227-1-RR (same clone as 83227-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 83227-1-RR (same clone as 83227-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83227-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00177-1

  Cytometric bead array standard curve of MP00177-1, TCP11L2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83227-1-PBS. Detection antibody: 83227-2-PBS. Standard: Ag11185. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 83227-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human TCP11L2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83227-1-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA TCP11L2 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TCP11L2 fusion protein Ag11185
• Vollständiger Name: t-complex 11 (mouse)-like 2
• Berechnetes Molekulargewicht: 519 aa, 58 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC033617
• Gene symbol: TCP11L2
• Gene ID (NCBI): 255394
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)