TCTN2 Rekombinanter Antikörper

TCTN2 Rekombinant Antikörper für IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6N22

Kat-Nr. : 82120-1-PBS

Synonyme

Tectonic-2, tectonic family member 2, Tectonic 2, TECT2, C12orf38

---

• 

  IHC staining of human colon cancer using 82120-1-RR (same clone as 82120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82120-1-RR (TCTN2 antibody) at dilution of 1:1050 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82120-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 82120-1-RR (same clone as 82120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82120-1-RR (TCTN2 antibody) at dilution of 1:1050 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82120-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82120-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82120-1-RR against Human TCTN2 were performed. The affinity constant is 66 nM.

---

Geprüfte Anwendungen

Produktinformation

82120-1-PBS bindet in IHC, Indirect ELISA TCTN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TCTN2 fusion protein Ag10725
• Vollständiger Name: tectonic family member 2
• Berechnetes Molekulargewicht: 697 aa, 77 kDa
• GenBank-Zugangsnummer: BC009112
• Gene symbol: TCTN2
• Gene ID (NCBI): 79867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.