TCTN2 Rekombinanter Antikörper

TCTN2 Rekombinant Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

6N22

Kat-Nr. : 82120-1-RR

Synonyme

Tectonic-2, tectonic family member 2, Tectonic 2, TECT2, C12orf38

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  IHC staining of human colon cancer using 82120-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82120-1-RR (TCTN2 antibody) at dilution of 1:1050 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 82120-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82120-1-RR (TCTN2 antibody) at dilution of 1:1050 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82120-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82120-1-RR against Human TCTN2 were performed. The affinity constant is 66 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:525-1:2100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82120-1-RR bindet in IHC, ELISA TCTN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TCTN2 fusion protein Ag10725
• Vollständiger Name: tectonic family member 2
• Berechnetes Molekulargewicht: 697 aa, 77 kDa
• GenBank-Zugangsnummer: BC009112
• Gene symbol: TCTN2
• Gene ID (NCBI): 79867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.