TCTN2 Rekombinanter Antikörper

TCTN2 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E17

Kat-Nr. : 82121-1-PBS

Synonyme

C12orf38, FLJ12975, TCTN2, TECT2, Tectonic 2, tectonic family member 2

---

• 

  WB analysis using 82121-1-RR (same clone as 82121-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82121-1-RR (TCTN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82121-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82121-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 82121-1-RR against Human TCTN2 were performed. The affinity constant is 4.48 nM.

---

Geprüfte Anwendungen

Produktinformation

82121-1-PBS bindet in WB, Indirect ELISA TCTN2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TCTN2 fusion protein Ag10725
• Vollständiger Name: tectonic family member 2
• Berechnetes Molekulargewicht: 697 aa, 77 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC009112
• Gene symbol: TCTN2
• Gene ID (NCBI): 79867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.