TCTN3 Polyklonaler Antikörper

TCTN3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 16085-1-AP

Synonyme

TECT3, TCTN 3, PSEC0041, OFD4, JBTS18

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  WB analysis using 16085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16085-1-AP (TCTN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Hela cells using 16085-1-AP

  IP result of anti-TCTN3 (16085-1-AP for IP and Detection).

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  IHC staining of human lung using 16085-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16085-1-AP (TCTN3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 16085-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16085-1-AP (TCTN3 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausembryogewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

16085-1-AP bindet in WB, IHC, IF, ELISA TCTN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TCTN3 fusion protein Ag9084
• Vollständiger Name: tectonic family member 3
• Berechnetes Molekulargewicht: 607 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC009494
• Gene symbol: TCTN3
• Gene ID (NCBI): 26123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TCTN3 (tectonic-3 or TECT3) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. TCTN3 is necessary for transduction of the sonic hedgehog (SHH) signaling pathway. Orofaciodigital syndromes (OFDSs) consist of a group of heterogeneous disorders characterized by abnormalities in the oral cavity, face, and digits and associated phenotypic abnormalities. Recently, TCTN3 mutations are found as the cause of an extreme form of OFD associated with bone dysplasia, tibial defect, cystic kidneys, and brain anomalies.

Publikationen

Species	Application	Title
human	WB,IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering
	WB	bioRxiv; Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis; Authors - Manu Ahmed